Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 22
Page 8-4
... immunoprecipitation and SDS - polyacrylamide gel electrophoresis . After autoradiography , the radioactive band can be excised from the gel and counted in a scintillation counter . The proportion of the total radioactivity contained in ...
... immunoprecipitation and SDS - polyacrylamide gel electrophoresis . After autoradiography , the radioactive band can be excised from the gel and counted in a scintillation counter . The proportion of the total radioactivity contained in ...
Page 8-51
... Immunoprecipitation of the polypeptide synthesized in vitro or in vivo from transcripts of the cDNA clone by antibodies raised against the protein of interest . The stringency of this test increases when it is carried out with a series ...
... Immunoprecipitation of the polypeptide synthesized in vitro or in vivo from transcripts of the cDNA clone by antibodies raised against the protein of interest . The stringency of this test increases when it is carried out with a series ...
Page 1-19
... immunoprecipitation . See Immunopre- cipitation solid - phase radioimmunoassay , 18.19- 18.20 using two antibodies , 18.21-18.23 western blotting . See Western blotting Immunological screening of bacterial colonies , 12.21-12.28 lysis ...
... immunoprecipitation . See Immunopre- cipitation solid - phase radioimmunoassay , 18.19- 18.20 using two antibodies , 18.21-18.23 western blotting . See Western blotting Immunological screening of bacterial colonies , 12.21-12.28 lysis ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector