Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-26
... length of the newly synthesized chains . Design the bacteriophage M13 or phagemid recombinant so that the probe will be as short as possible . If the average length of the DNA synthesized during the first stage of the reaction ( i.e. ...
... length of the newly synthesized chains . Design the bacteriophage M13 or phagemid recombinant so that the probe will be as short as possible . If the average length of the DNA synthesized during the first stage of the reaction ( i.e. ...
Page 11-8
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
... length ( which for screening of a typical mammalian cDNA library is 17-18 nucleotides [ Lathe 1985 ] ) , it is worthwhile increasing its length in order to maximize its specificity . The concomitant increase in mismatches that occurs as ...
Page 13-45
... length of the gel . A length of 40-50 cm is standard , but apparatuses that accommodate gels up to 100 cm in length are available . However , long gels are extremely difficult to handle and require special , large containers for ...
... length of the gel . A length of 40-50 cm is standard , but apparatuses that accommodate gels up to 100 cm in length are available . However , long gels are extremely difficult to handle and require special , large containers for ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector