Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 85
Page 8-24
... ligation of two unrelated cDNAs . The ligation mixture should therefore always contain a high molar excess of vector DNA to cDNA . However , these conditions strongly favor re - formation of the vector by self - ligation , leading to ...
... ligation of two unrelated cDNAs . The ligation mixture should therefore always contain a high molar excess of vector DNA to cDNA . However , these conditions strongly favor re - formation of the vector by self - ligation , leading to ...
Page 13-24
... Ligation of the Target DNA 1. Using the appropriate restriction enzymes , digest a sufficient amount of recombinant ... ligation conditions as described in the note to step 2. If it is not possible to avoid restriction enzymes that ...
... Ligation of the Target DNA 1. Using the appropriate restriction enzymes , digest a sufficient amount of recombinant ... ligation conditions as described in the note to step 2. If it is not possible to avoid restriction enzymes that ...
Page 15-10
... ligation buffer amount calculated above 100 ng 2 μ . blunt - end ligation buffer should be added last to reduce the possibility that the DNA will be precipitated by spermidine . Add 1-2 Weiss units of bacteriophage T4 DNA ligase , and ...
... ligation buffer amount calculated above 100 ng 2 μ . blunt - end ligation buffer should be added last to reduce the possibility that the DNA will be precipitated by spermidine . Add 1-2 Weiss units of bacteriophage T4 DNA ligase , and ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector