Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 50
Page 15-5
... linear molecules ( Melgar and Goldthwait 1968 ) . These unit- length linear molecules are purified to remove circular plasmid DNA , recircularized in the presence of a synthetic linker containing a restriction enzyme cleavage site that ...
... linear molecules ( Melgar and Goldthwait 1968 ) . These unit- length linear molecules are purified to remove circular plasmid DNA , recircularized in the presence of a synthetic linker containing a restriction enzyme cleavage site that ...
Page 15-8
... linear plasmid is detectable , a new preparation should be made ( see Chapter 1 ) . The major problem with this method is the generation of full - length linear molecules after partial digestion with a restriction enzyme that cleaves ...
... linear plasmid is detectable , a new preparation should be made ( see Chapter 1 ) . The major problem with this method is the generation of full - length linear molecules after partial digestion with a restriction enzyme that cleaves ...
Page 15-9
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mM EDTA ( pH 8.0 ) to each tube to ...
... linear DNA will be used as an electrophoretic marker ( see h ) . g . After the eight test reactions have incubated for 30 minutes , quickly transfer the tubes to an ice - water bath . Add 5 μl of 50 mM EDTA ( pH 8.0 ) to each tube to ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector