Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 68
Page 8-27
... linkers , and the hairpin loop is cleaved with nuclease S1 and repaired with the Klenow fragment of E. coli DNA polymerase I before linkers of a second type are ligated to the cDNA . These linkers will be added to both ends of the cDNA ...
... linkers , and the hairpin loop is cleaved with nuclease S1 and repaired with the Klenow fragment of E. coli DNA polymerase I before linkers of a second type are ligated to the cDNA . These linkers will be added to both ends of the cDNA ...
Page 15-10
... linker ( in nucleotides ) , y = length of linear plasmid DNA ( in nucleotides ) , and z = desired molar ratio of linkers to target DNA . Usually , a 20- to 30 - fold molar excess of linkers is used to ensure that several copies of the ...
... linker ( in nucleotides ) , y = length of linear plasmid DNA ( in nucleotides ) , and z = desired molar ratio of linkers to target DNA . Usually , a 20- to 30 - fold molar excess of linkers is used to ensure that several copies of the ...
Page 15-11
... linkers with the appropriate restriction enzyme ( s ) to remove polymers of linkers from the termini of the linearized plasmid DNA and to create cohesive termini . Either of two methods can be used to digest the linkers . In the first ...
... linkers with the appropriate restriction enzyme ( s ) to remove polymers of linkers from the termini of the linearized plasmid DNA and to create cohesive termini . Either of two methods can be used to digest the linkers . In the first ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector