Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-38
... lysogens efficiently at 32 ° C . In the original description of this vector ( Young and Davis 1983a ) , libraries of ... lysogenic colonies , are screened with immuno- logical probes ( Young and Davis 1983b ) . Libraries constructed in ...
... lysogens efficiently at 32 ° C . In the original description of this vector ( Young and Davis 1983a ) , libraries of ... lysogenic colonies , are screened with immuno- logical probes ( Young and Davis 1983b ) . Libraries constructed in ...
Page 12-38
... Lysogens The isolation of recombinant bacteriophages encoding fusion proteins that bind to double - stranded ... lysogenic for recombinant bacteriophage Agt11 . Usually 10-70 % of the colonies tested are lysogens . 7. Inoculate 2 ...
... Lysogens The isolation of recombinant bacteriophages encoding fusion proteins that bind to double - stranded ... lysogenic for recombinant bacteriophage Agt11 . Usually 10-70 % of the colonies tested are lysogens . 7. Inoculate 2 ...
Page 12-39
... lysogens are induced . 10. Transfer the cultures to a water bath equilibrated to 44 ° C . Incubate the cultures for ... lysogenic cultures to two microfuge tubes . Immediately centrifuge the tubes at 12,000g for 30 seconds at 4 ° C in a ...
... lysogens are induced . 10. Transfer the cultures to a water bath equilibrated to 44 ° C . Incubate the cultures for ... lysogenic cultures to two microfuge tubes . Immediately centrifuge the tubes at 12,000g for 30 seconds at 4 ° C in a ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector