Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-4
... mRNA from which it is derived , it is important to check the integrity of the preparation of mRNA before it is used as the template for synthesis of the first strand of cDNA . The following tests are commonly used : • The ability of the ...
... mRNA from which it is derived , it is important to check the integrity of the preparation of mRNA before it is used as the template for synthesis of the first strand of cDNA . The following tests are commonly used : • The ability of the ...
Page 8-6
... mRNA preparations that are rich in the sequences of interest , or of mRNA that has been partially fragmented by limited alkaline hydrolysis and end - labeled by phosphor- ylation . As a good approximation , the mRNA sequences of ...
... mRNA preparations that are rich in the sequences of interest , or of mRNA that has been partially fragmented by limited alkaline hydrolysis and end - labeled by phosphor- ylation . As a good approximation , the mRNA sequences of ...
Page 8-48
... mRNA is depleted of sequences that are present in a second type of mRNA by subtractive hybridization ( Timberlake 1980 ; Zimmerman et al . 1980 ) . Typically , the cDNA is hybridized two or three times in succession to a 20 - fold ...
... mRNA is depleted of sequences that are present in a second type of mRNA by subtractive hybridization ( Timberlake 1980 ; Zimmerman et al . 1980 ) . Typically , the cDNA is hybridized two or three times in succession to a 20 - fold ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector