Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 96
Page 9-32
... mammalian genomic DNA , approxi- mately 10 μg of DNA should be loaded into each slot of the gel when probes of standard length ( > 500 bp ) and high specific activity ( > 109 cpm / μg ) are used to detect single - copy sequences ; 30-50 ...
... mammalian genomic DNA , approxi- mately 10 μg of DNA should be loaded into each slot of the gel when probes of standard length ( > 500 bp ) and high specific activity ( > 109 cpm / μg ) are used to detect single - copy sequences ; 30-50 ...
Page 1-19
... mammalian cells , 3.19 in mammalian expression vector pHyg , 16.13 as selectable marker in mammalian cells , 16.14 Hypoxanthine as selective agent , 16.9 . See also HAT medium structure , C.12 Hypoxanthine - guanine phosphoribosyl ...
... mammalian cells , 3.19 in mammalian expression vector pHyg , 16.13 as selectable marker in mammalian cells , 16.14 Hypoxanthine as selective agent , 16.9 . See also HAT medium structure , C.12 Hypoxanthine - guanine phosphoribosyl ...
Page 1-36
... mammalian expression vectors , 16.5-16.6 . See also Mammalian cells , expression vectors for synthesis of RNA probes . See Bacteriophage - encoded DNA- dependent RNA polymerases TATA box , 16.6 upstream promoter elements , 16.6 Pronase ...
... mammalian expression vectors , 16.5-16.6 . See also Mammalian cells , expression vectors for synthesis of RNA probes . See Bacteriophage - encoded DNA- dependent RNA polymerases TATA box , 16.6 upstream promoter elements , 16.6 Pronase ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector