Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-6
... method was too inaccurate and clumsy to gain general acceptance , and it was not until the introduction of chain - terminating dideoxynucleoside triphosphates ( ddNTPs ) ( Sanger et al . 1977 ) that en- zymatic methods of DNA sequencing ...
... method was too inaccurate and clumsy to gain general acceptance , and it was not until the introduction of chain - terminating dideoxynucleoside triphosphates ( ddNTPs ) ( Sanger et al . 1977 ) that en- zymatic methods of DNA sequencing ...
Page 13-11
... METHOD Unlike the chain - termination technique , which involves synthesis , the Maxam - Gilbert method involves chemical degradation of the original DNA . This method grew out of studies of the interaction between the lac repressor and ...
... METHOD Unlike the chain - termination technique , which involves synthesis , the Maxam - Gilbert method involves chemical degradation of the original DNA . This method grew out of studies of the interaction between the lac repressor and ...
Page 1-6
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
... method , 16.37 cells growing in suspension , method , 16.38 modified ( Chen and Okayama ) method , 16.39-16.40 Calf intestinal alkaline phosphatase . See CIP Capillary transfer of nucleic acids from agarose gels to solid supports . See ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector