Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 13
Page xii
... METHYLASE 5.15 dcm METHYLASE 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli 5.15 M.EcoRI METHYLASE 5.16 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA ...
... METHYLASE 5.15 dcm METHYLASE 5.15 METHYLATION - DEPENDENT RESTRICTION SYSTEMS IN E. coli 5.15 M.EcoRI METHYLASE 5.16 Modification of Restriction Sites by DNA Methylation 5.16 Influence of Methylation on DNA Mapping 5.26 DIGESTING DNA ...
Page 8-39
... methylases to protect potential sites of cleavage with the restriction enzymes BamHI and HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase and M.AluI methylase ( which ...
... methylases to protect potential sites of cleavage with the restriction enzymes BamHI and HindIII . Because M.HindIII methylase is not commercially available , the cDNA is methylated with M.BamHI methylase and M.AluI methylase ( which ...
Page 1-38
... methylase , 2.57 , 5.15 dcm methylase , 5.15 deficient strains for growing bacterio- phage λ , 2.55 EcoK is missing from E. coli C , 2.99 , 5.15 EcoRI methylase , 5.16 treatment of cDNA , 8.24 , 8.66–8.67 hsdM , genetic marker in E ...
... methylase , 2.57 , 5.15 dcm methylase , 5.15 deficient strains for growing bacterio- phage λ , 2.55 EcoK is missing from E. coli C , 2.99 , 5.15 EcoRI methylase , 5.16 treatment of cDNA , 8.24 , 8.66–8.67 hsdM , genetic marker in E ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector