Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 63
Page 11-36
... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to thaw at room temperature . 4. Centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . Using a pasteur ...
... tube in a dry - ice / methanol bath until the mixture is frozen . Remove the tube from the bath , and allow the mixture to thaw at room temperature . 4. Centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . Using a pasteur ...
Page 13-26
... microfuge tube . Store this tube on ice . Between bursts , centrifuge the original mi- crofuge tube for 2 seconds to deposit the DNA solution at the bottom of the tube , and add fresh ice to the sonicator horn to maintain the ...
... microfuge tube . Store this tube on ice . Between bursts , centrifuge the original mi- crofuge tube for 2 seconds to deposit the DNA solution at the bottom of the tube , and add fresh ice to the sonicator horn to maintain the ...
Page 14-14
... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the microfuge locat- ed in the laminar flow hood . This deposits the fluid in the base of the tube and reduces the ...
... microfuge tubes containing reagents used in polymerase chain reactions , centrifuge them briefly ( 10 seconds ) in the microfuge locat- ed in the laminar flow hood . This deposits the fluid in the base of the tube and reduces the ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector