Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-23
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
... molecules . The reaction often proceeds asynchronously , so that different termini of DNA molecules carry homopolymeric tails of very different lengths . This prob- lem can be alleviated , but not completely solved , by using plasmids ...
Page 8-24
... molecules with cohesive termini . These molecules are then purified and ligated to a large molar excess of the appropriate dephosphorylated vector . • The use of synthetic adapters rather than linkers . Adapters are short , double ...
... molecules with cohesive termini . These molecules are then purified and ligated to a large molar excess of the appropriate dephosphorylated vector . • The use of synthetic adapters rather than linkers . Adapters are short , double ...
Page 15-27
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
... molecules . Few of the molecules are cleaved more than once by the enzyme . Digestion with a restriction enzyme that cleaves at one end of the target sequence therefore generates a mixed population that contains full - length linear ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector