Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 15-54
... oligonucleotide will hybridize to its target sequence in the template DNA . In extremely rare cases , the secondary structure of the oligonucleotide ( or ... Mutagenesis of Cloned DNA Design and Selection of Mutagenic Oligonucleotides 15 54.
... oligonucleotide will hybridize to its target sequence in the template DNA . In extremely rare cases , the secondary structure of the oligonucleotide ( or ... Mutagenesis of Cloned DNA Design and Selection of Mutagenic Oligonucleotides 15 54.
Page 15-55
... mutagenic primer from its template . • The hybrid formed between the template and the 3 ' terminus of the oligonucleotide ... mutagenic oligonucleotide is now free to anneal to incorrect sites on the template . To suppress these effects ...
... mutagenic primer from its template . • The hybrid formed between the template and the 3 ' terminus of the oligonucleotide ... mutagenic oligonucleotide is now free to anneal to incorrect sites on the template . To suppress these effects ...
Page 15-80
... oligonucleotide - mediated , site - directed mutagenesis , along with suggested solutions for these problems . 1. Plaques or colonies are obtained that hybridize specifically to the mutagenic ... mutagenic oligonucleotide by polyacrylamide ...
... oligonucleotide - mediated , site - directed mutagenesis , along with suggested solutions for these problems . 1. Plaques or colonies are obtained that hybridize specifically to the mutagenic ... mutagenic oligonucleotide by polyacrylamide ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector