Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xviii
... Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO NITROCELLULOSE FILTERS 9.41 Transfer of DNA from Agarose Gels to Nylon Membranes 9.42 ...
... Nitrocellulose Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO NITROCELLULOSE FILTERS 9.41 Transfer of DNA from Agarose Gels to Nylon Membranes 9.42 ...
Page 9-38
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Transfer of DNA to Nitrocellulose Filters CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 1. After electrophoresis , transfer the gel to a glass baking dish and trim away any unused ...
Page 9-41
... NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the gel for transfer ...
... NITROCELLULOSE FILTERS DNA can be transferred simultaneously from a single agarose gel to two nitrocellulose filters as described by Smith and Summers ( 1980 ) ( see Figure 9.4 ) . 1. After electrophoresis , prepare the gel for transfer ...
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector