Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xviii
... Nylon Membranes 9.42 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 9.44 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER ALKALINE CONDITIONS 9.45 HYBRIDIZATION OF RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ...
... Nylon Membranes 9.42 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 9.44 CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER ALKALINE CONDITIONS 9.45 HYBRIDIZATION OF RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ...
Page 9-42
... Nylon Membranes For almost 20 years , immobilization and hybridization of denatured DNA was carried out exclusively with nitrocellulose - first with powder ( Hall and Spiegelman 1961 ) and then with sheets ( Nygaard ... Nylon Membranes 9 42.
... Nylon Membranes For almost 20 years , immobilization and hybridization of denatured DNA was carried out exclusively with nitrocellulose - first with powder ( Hall and Spiegelman 1961 ) and then with sheets ( Nygaard ... Nylon Membranes 9 42.
Page 9-44
A Laboratory Manual Joseph Sambrook, Tom Maniatis. CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 1. Fractionate the DNA by gel electrophoresis , and process the gel as described in ... NYLON MEMBRANES UNDER NEUTRAL.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. CAPILLARY TRANSFER OF DNA TO NYLON MEMBRANES UNDER NEUTRAL CONDITIONS 1. Fractionate the DNA by gel electrophoresis , and process the gel as described in ... NYLON MEMBRANES UNDER NEUTRAL.
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector