Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 86
Page 11-21
... oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex ... Oligonucleotide Probes 11.21 PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES 11 21 Preparation of Synthetic Oligonucleotides 11 21.
... oligonucleotide ( usually 1-10 nmoles ) in 1 ml of sterile , filtered H2O ( Milli - Q or equivalent ) . Vortex ... Oligonucleotide Probes 11.21 PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES 11 21 Preparation of Synthetic Oligonucleotides 11 21.
Page 11-30
... oligonucleotide applied to the column should elute in the first fraction . 5. Evaporate ... oligonucleotide LABELING OF SYNTHETIC OLIGONUCLEOTIDES BY PHOSPHORYLATION WITH BACTERIOPHAGE T4 POLYNUCLEOTIDE 11.30 Synthetic Oligonucleotide Probes.
... oligonucleotide applied to the column should elute in the first fraction . 5. Evaporate ... oligonucleotide LABELING OF SYNTHETIC OLIGONUCLEOTIDES BY PHOSPHORYLATION WITH BACTERIOPHAGE T4 POLYNUCLEOTIDE 11.30 Synthetic Oligonucleotide Probes.
Page 15-80
... oligonucleotide by polyacrylamide gel electro- phoresis . • Test the mutagenic oligonucleotide for its ability to prime DNA synthe- sis at the appropriate location on the single - stranded template DNA ( Zoller and Smith 1987 ) . Use ...
... oligonucleotide by polyacrylamide gel electro- phoresis . • Test the mutagenic oligonucleotide for its ability to prime DNA synthe- sis at the appropriate location on the single - stranded template DNA ( Zoller and Smith 1987 ) . Use ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector