Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 62
Page 8-73
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
... plaques on strains of E. coli such as Y1090hsdR in the presence of the inducer IPTG and the chromogenic substrate X - gal ; recombinant plaques are colorless . Nonrecombinant bacteriophages do not present a problem when bac- teriophages ...
Page 12-16
... plaques is low . The color produced by the chromogenic reaction is most intense at the expanding edge of the plaque . Well - isolated immunopositive plaques therefore display a characteristic ringlike pattern . When plaques crowd ...
... plaques is low . The color produced by the chromogenic reaction is most intense at the expanding edge of the plaque . Well - isolated immunopositive plaques therefore display a characteristic ringlike pattern . When plaques crowd ...
Page 15-70
... plaques usually contain a mixture of both mutant and wild - type sequences . It is therefore essential to plaque - purify the bacteriophages from positively hybridizing plaques as follows : a . Touch the blunt end of a sterile ...
... plaques usually contain a mixture of both mutant and wild - type sequences . It is therefore essential to plaque - purify the bacteriophages from positively hybridizing plaques as follows : a . Touch the blunt end of a sterile ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector