Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 14-14
... polymerase chain reactions have been set up . Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for 14.14 In Vitro Amplification of DNA by the Polymerase Chain Reaction AMPLIFICATION METHODS 14 14 14.
... polymerase chain reactions have been set up . Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for 14.14 In Vitro Amplification of DNA by the Polymerase Chain Reaction AMPLIFICATION METHODS 14 14 14.
Page 14-15
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for priming the ... Polymerase Chain Reaction 14 15 15 BUFFERS USED FOR POLYMERASE CHAIN REACTIONS 14 15.
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Components of the Polymerase Chain Reaction OLIGONUCLEOTIDES Oligonucleotides used for priming the ... Polymerase Chain Reaction 14 15 15 BUFFERS USED FOR POLYMERASE CHAIN REACTIONS 14 15.
Page 14-16
... reaction , and any change in their concentration affects the concentration of available Mg ++ . We recommend setting up a set ... Polymerase Chain Reaction Taq DNA POLYMERASE 14 16 DEOXYRIBONUCLEOSIDE TRIPHOSPHATES 14 16 TARGET SEQUENCES 16.
... reaction , and any change in their concentration affects the concentration of available Mg ++ . We recommend setting up a set ... Polymerase Chain Reaction Taq DNA POLYMERASE 14 16 DEOXYRIBONUCLEOSIDE TRIPHOSPHATES 14 16 TARGET SEQUENCES 16.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector