Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 46
Page 11-9
... position of codon U G AG ט כ AG 3rd position of codon ( 3 ' terminus ) U C A G บ บ บ Phe UUC Phe UCU Ser UCC Ser UAU Tyr UGU Cys UAC Tyr UGC Cys U UUA Leu UCA Ser UAA Stop ( Ochre ) UGA Stop ( Umber ) UUG Leu UCG Ser UAG Stop ( Amber ) ...
... position of codon U G AG ט כ AG 3rd position of codon ( 3 ' terminus ) U C A G บ บ บ Phe UUC Phe UCU Ser UCC Ser UAU Tyr UGU Cys UAC Tyr UGC Cys U UUA Leu UCA Ser UAA Stop ( Ochre ) UGA Stop ( Umber ) UUG Leu UCG Ser UAG Stop ( Amber ) ...
Page 11-11
... position ( " guessmers " ) . In many cases , only a single oligonucleotide 30-70 nucleotides in length is synthesized that contains the combination of codons most likely to match the authentic gene . Hybridization with oligonucleotides ...
... position ( " guessmers " ) . In many cases , only a single oligonucleotide 30-70 nucleotides in length is synthesized that contains the combination of codons most likely to match the authentic gene . Hybridization with oligonucleotides ...
Page 11-17
... position of codons that code for the same amino acid and differ by A / G in the third position ( e.g. , Glu : GAA / GAG ) . However , the use of inosine in codons that differ by T / C in their third position is of less value ...
... position of codons that code for the same amino acid and differ by A / G in the third position ( e.g. , Glu : GAA / GAG ) . However , the use of inosine in codons that differ by T / C in their third position is of less value ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector