Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 86
Page 11-10
... possible to avoid incorporating the two incorrect codons TTT and TTC ( both of which code for Phe ) into the oligonucleotide . 3. If enough amino acid sequence is available , it may be possible to design nonoverlapping or partially ...
... possible to avoid incorporating the two incorrect codons TTT and TTC ( both of which code for Phe ) into the oligonucleotide . 3. If enough amino acid sequence is available , it may be possible to design nonoverlapping or partially ...
Page 11-15
... possible , and often desirable , to synthesize a small pool of 2-8 guessmers that contains all possible choices at certain positions . It is best to carry out this kind of limited substitution when an amino acid whose codon is highly ...
... possible , and often desirable , to synthesize a small pool of 2-8 guessmers that contains all possible choices at certain positions . It is best to carry out this kind of limited substitution when an amino acid whose codon is highly ...
Page 13-82
... possible to selectively label a designated terminus with the appropriate [ 32P ] dNTP and the Klenow fragment of E. coli DNA polymerase I. In the example shown in Figure 13.11 , a recombinant plasmid carrying the target at the central ...
... possible to selectively label a designated terminus with the appropriate [ 32P ] dNTP and the Klenow fragment of E. coli DNA polymerase I. In the example shown in Figure 13.11 , a recombinant plasmid carrying the target at the central ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector