Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 48
Page 8-55
... precipitated by ethanol . • Save the ethanol solution until you are sure that all of the DNA has been recovered . This is especially important after precipitates of DNA have been washed with 70 % ethanol - treatment that often loosens ...
... precipitated by ethanol . • Save the ethanol solution until you are sure that all of the DNA has been recovered . This is especially important after precipitates of DNA have been washed with 70 % ethanol - treatment that often loosens ...
Page 10-65
... precipitate to dry completely at room temperature before dissolving it in TE ( pH 7.6 ) at a concentration of more ... precipitates from solution if its concentration exceeds 5-10 mм . ii . Until recently , CIP was supplied as a ...
... precipitate to dry completely at room temperature before dissolving it in TE ( pH 7.6 ) at a concentration of more ... precipitates from solution if its concentration exceeds 5-10 mм . ii . Until recently , CIP was supplied as a ...
Page 11-35
... precipitate radiolabeled nucleic acids , including oligonucleotides , from labeling reactions . The nucleic acids are precipitated from aqueous solution with the cationic detergent cetylpyridinium bromide ( CPB ) . The detergent is then ...
... precipitate radiolabeled nucleic acids , including oligonucleotides , from labeling reactions . The nucleic acids are precipitated from aqueous solution with the cationic detergent cetylpyridinium bromide ( CPB ) . The detergent is then ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector