Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 89
Page 9-7
... prepare the genomic DNA for cloning and to recover fragments from specific recombinants . • The ease with which arms can be prepared . A few bacteriophage λ vectors have been designed to allow recombinants to be selected by growth in ...
... prepare the genomic DNA for cloning and to recover fragments from specific recombinants . • The ease with which arms can be prepared . A few bacteriophage λ vectors have been designed to allow recombinants to be selected by growth in ...
Page 10-18
... prepared in vitro from DNA templates by ( 1 ) synthesis of radiolabeled DNA that is complementary to sequences ... Preparation of Radiolabeled DNA and RNA Probes Preparation of Single-stranded Probes 10 18.
... prepared in vitro from DNA templates by ( 1 ) synthesis of radiolabeled DNA that is complementary to sequences ... Preparation of Radiolabeled DNA and RNA Probes Preparation of Single-stranded Probes 10 18.
Page 13-59
... prepared as described on page 13.60 . 3. Pour the required number of polyacrylamide sequencing gels as described on pages 13.45-13.54 . PREPARATION OF WORKING SOLUTIONS OF dNTPs 1. Prepare working solutions DNA Sequencing 13.59.
... prepared as described on page 13.60 . 3. Pour the required number of polyacrylamide sequencing gels as described on pages 13.45-13.54 . PREPARATION OF WORKING SOLUTIONS OF dNTPs 1. Prepare working solutions DNA Sequencing 13.59.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector