Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-48
... probe added ( in micrograms ) , y = its complex- ity ( for most probes , complexity is proportional to the length of the probe in kilobases ) , and z = the volume of the reaction ( in milliliters ) . After hybridization to 3 × Cot1 / 2 ...
... probe added ( in micrograms ) , y = its complex- ity ( for most probes , complexity is proportional to the length of the probe in kilobases ) , and z = the volume of the reaction ( in milliliters ) . After hybridization to 3 × Cot1 / 2 ...
Page 9-53
... probe from binding to T - rich sequences that are found fairly commonly in eukaryotic DNA . 2. Float the ... probe is double - stranded , denature it by heating for 5 minutes at 100 ° C . Single - stranded probe need not be denatured ...
... probe from binding to T - rich sequences that are found fairly commonly in eukaryotic DNA . 2. Float the ... probe is double - stranded , denature it by heating for 5 minutes at 100 ° C . Single - stranded probe need not be denatured ...
Page 10-18
... probes are more stable than those formed between the probe and the target sequences . The resulting segregation of the probe leads to a decrease in the sensitivity with which the target sequences can be detected . The absence of a ...
... probes are more stable than those formed between the probe and the target sequences . The resulting segregation of the probe leads to a decrease in the sensitivity with which the target sequences can be detected . The absence of a ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector