Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 97
Page xviii
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page 10-18
... probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes ... Probes Preparation of Single-stranded Probes 10 18.
... probes , which consist of only one of the two complementary strands of a given sequence of nucleic acid , have a considerable advantage over the more traditional double - stranded probes ... Probes Preparation of Single-stranded Probes 10 18.
Page 10-27
... probes of high specific activity was the development of plasmid vectors containing polycloning sites downstream from powerful promoters derived from the Salmonella ... probes has led to Preparation of Radiolabeled DNA and RNA Probes 10.27.
... probes of high specific activity was the development of plasmid vectors containing polycloning sites downstream from powerful promoters derived from the Salmonella ... probes has led to Preparation of Radiolabeled DNA and RNA Probes 10.27.
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agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector