Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-80
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PROBLEMS COMMONLY ENCOUNTERED WITH CDNA CLONING Problems commonly ... problem still persists , check the other components of the first - strand reaction , using an mRNA template whose ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. PROBLEMS COMMONLY ENCOUNTERED WITH CDNA CLONING Problems commonly ... problem still persists , check the other components of the first - strand reaction , using an mRNA template whose ...
Page 13-74
... problems are listed in Table 13.5 together with suggestions for solving them . When problems of this type arise , it is ... problem persists , try substituting the base analog dITP for dGTP in the chain - extension / chain - termination ...
... problems are listed in Table 13.5 together with suggestions for solving them . When problems of this type arise , it is ... problem persists , try substituting the base analog dITP for dGTP in the chain - extension / chain - termination ...
Page 15-80
... problem regions . Use a shorter oligonucleotide as a hybridization probe to screen for the desired mutant . 3. All plaques or colonies hybridize to the oligonucleotide probe . • Check that there is no region of extensive homology ...
... problem regions . Use a shorter oligonucleotide as a hybridization probe to screen for the desired mutant . 3. All plaques or colonies hybridize to the oligonucleotide probe . • Check that there is no region of extensive homology ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector