Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 88
Page 12-2
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
... proteins in recombinant libraries constructed in expression vectors . In every case , these methods involve screening the library with a ligand that binds specifically to the protein of interest . Such ligands fall into three classes ...
Page 12-3
... protein is malfolded or denatured , antibodies are the reagents of choice for primary screening of expression ... protein ( Walter et al . 1985 ; Kadonaga et al . 1987 ; Bodner et al . 1988 ) or ( 2 ) screening with antibodies raised ...
... protein is malfolded or denatured , antibodies are the reagents of choice for primary screening of expression ... protein ( Walter et al . 1985 ; Kadonaga et al . 1987 ; Bodner et al . 1988 ) or ( 2 ) screening with antibodies raised ...
Page 15-81
... protein and ( 2 ) X - ray diffraction of protein crystals . Although both of these methods have yielded much information , they can be used only with proteins that are available in large quantity and are of high purity . A less direct ...
... protein and ( 2 ) X - ray diffraction of protein crystals . Although both of these methods have yielded much information , they can be used only with proteins that are available in large quantity and are of high purity . A less direct ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector