Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 87
Page 8-62
... purified from particles of avian ret- roviruses : poly ( A ) + RNA ( 1 mg / ml ) 10.0 με oligo ( dT ) 12-18 ( 1 mg / ml ) 10.0 μl • 1 м Tris Cl ( pH 8.3 ) at 42 ° C 2.5 μl 1 M KCl 7.0 με 250 mM MgCl2 2.0 με mixture of dNTPs ( 5 mм each ) ...
... purified from particles of avian ret- roviruses : poly ( A ) + RNA ( 1 mg / ml ) 10.0 με oligo ( dT ) 12-18 ( 1 mg / ml ) 10.0 μl • 1 м Tris Cl ( pH 8.3 ) at 42 ° C 2.5 μl 1 M KCl 7.0 με 250 mM MgCl2 2.0 με mixture of dNTPs ( 5 mм each ) ...
Page 12-13
... purified by affinity chromatography on columns of the immobilized antigen . Note that affinity purification must be carried out with an independent preparation of antigen that is free of the contaminants present in the material used for ...
... purified by affinity chromatography on columns of the immobilized antigen . Note that affinity purification must be carried out with an independent preparation of antigen that is free of the contaminants present in the material used for ...
Page 13-24
... purified target DNA . Separate the target DNA from the vector by preparative gel electrophoresis , and recover the target DNA from the gel by one of the methods described in Chapter 6. Redissolve the purified DNA in 15 μl of TE ( pH 7.6 ) ...
... purified target DNA . Separate the target DNA from the vector by preparative gel electrophoresis , and recover the target DNA from the gel by one of the methods described in Chapter 6. Redissolve the purified DNA in 15 μl of TE ( pH 7.6 ) ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector