Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 68
Page 10-2
... radioactive precursors ( usually 32P ) were introduced into cells that were synthesizing the DNA of interest . This procedure required large amounts of radioactivity , involved laborious purifi- cation of the nucleic acids of interest ...
... radioactive precursors ( usually 32P ) were introduced into cells that were synthesizing the DNA of interest . This procedure required large amounts of radioactivity , involved laborious purifi- cation of the nucleic acids of interest ...
Page 11-44
... radioactive marks on the labels as guides , locate the position of the probe in the gel . Excise the band and recover the radioactive oligonucleotide as described in Chapter 6 , pages 6.46-6.47 , starting at step 3 and ending at step 8 ...
... radioactive marks on the labels as guides , locate the position of the probe in the gel . Excise the band and recover the radioactive oligonucleotide as described in Chapter 6 , pages 6.46-6.47 , starting at step 3 and ending at step 8 ...
Page 15-69
... radioactive ink to the outside of the package and establish an au- toradiograph by exposing the package of filters to X - ray film for 1-2 hours at -70 ° C , using an intensifying screen ( see Appendix E ) . Radioactive ink is made by ...
... radioactive ink to the outside of the package and establish an au- toradiograph by exposing the package of filters to X - ray film for 1-2 hours at -70 ° C , using an intensifying screen ( see Appendix E ) . Radioactive ink is made by ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector