Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 92
Page xviii
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
... RADIOLABELED PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 ...
Page 10-15
... radiolabel . Because the reaction is carried out under conditions that minimize exonucleolytic digestion , the radiolabeled product is not degraded to a detectable extent . If necessary , therefore , the reaction can be allowed to ...
... radiolabel . Because the reaction is carried out under conditions that minimize exonucleolytic digestion , the radiolabeled product is not degraded to a detectable extent . If necessary , therefore , the reaction can be allowed to ...
Page 11-34
... radiolabeled oligonucleotide by centrifugation at 12,000g for 20 minutes at 0 ° C in a microfuge . Using an ... Radiolabeled Oligonucleotides by Precipitation with 34 Purification of Radiolabeled Oligonucleotides by Precipitation with.
... radiolabeled oligonucleotide by centrifugation at 12,000g for 20 minutes at 0 ° C in a microfuge . Using an ... Radiolabeled Oligonucleotides by Precipitation with 34 Purification of Radiolabeled Oligonucleotides by Precipitation with.
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector