Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 6
Page 8-23
... recA * , yields ten times as many recombinant cDNA clones made by the dA : dT tailing procedure as the recA strain HB101 yields ( Peacock et al . 1981 ) . Un- treated pBR322 DNA transforms the two strains at equal efficiency . These ...
... recA * , yields ten times as many recombinant cDNA clones made by the dA : dT tailing procedure as the recA strain HB101 yields ( Peacock et al . 1981 ) . Un- treated pBR322 DNA transforms the two strains at equal efficiency . These ...
Page 9-8
... recA bacteria , therefore , the rolling - circle DNA concatemers of gam recombinants are not degraded and production of progeny bacteriophage particles is not suppressed . The ability to propagate genomic DNA libraries in recA hosts ...
... recA bacteria , therefore , the rolling - circle DNA concatemers of gam recombinants are not degraded and production of progeny bacteriophage particles is not suppressed . The ability to propagate genomic DNA libraries in recA hosts ...
Page 1-14
... recA , recBC , and recF pathways , 2.56-2.57 mutations , 2.95-2.96 RecA protein , 5.88 recA1 strains for growth of bacterio- phage M13 and cosmid vectors , 3.6 , 4.13 recB and recC genes , 2.5 , 2.57 recD mutation and Spi bacterio ...
... recA , recBC , and recF pathways , 2.56-2.57 mutations , 2.95-2.96 RecA protein , 5.88 recA1 strains for growth of bacterio- phage M13 and cosmid vectors , 3.6 , 4.13 recB and recC genes , 2.5 , 2.57 recD mutation and Spi bacterio ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector