Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-73
... recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ligation reactions need to be carefully arranged so as to minimize the chance that a single recombinant bacteriophage will contain more than one ...
... recombinant bacteriophages ( i.e. , a mammalian cDNA library of reasonable size ) . The ligation reactions need to be carefully arranged so as to minimize the chance that a single recombinant bacteriophage will contain more than one ...
Page 8-75
... recombinant and nonrecombinant plaques . Ligation A should yield no recombinant plaques , whereas ligations B , C , and D should yield increasing numbers of recombinant plaques . From the number of recombinant plaques , calculate the ...
... recombinant and nonrecombinant plaques . Ligation A should yield no recombinant plaques , whereas ligations B , C , and D should yield increasing numbers of recombinant plaques . From the number of recombinant plaques , calculate the ...
Page 9-30
... recombinant bacteriophages in the packaging mixture directly for those that contain the DNA sequences of interest . If , for example , the recombinant you seek grows poorly , it may be heavily underrepresented in the amplified library ...
... recombinant bacteriophages in the packaging mixture directly for those that contain the DNA sequences of interest . If , for example , the recombinant you seek grows poorly , it may be heavily underrepresented in the amplified library ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector