Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 28
Page v
... Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical Identification of Recombinant Clones 1.8 Plasmid ...
... Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical Identification of Recombinant Clones 1.8 Plasmid ...
Page 1-25
... replication , 4.3-4.6 restriction enzyme digestion analysis , 4.28 small - scale preparation analysis of recombinants , 4.41 procedure , 4.26-4.28 DNA , single - stranded for DNA - sequencing reactions , 4.29– 4.30 in situ hybridization ...
... replication , 4.3-4.6 restriction enzyme digestion analysis , 4.28 small - scale preparation analysis of recombinants , 4.41 procedure , 4.26-4.28 DNA , single - stranded for DNA - sequencing reactions , 4.29– 4.30 in situ hybridization ...
Page 1-37
... replicating bacterial colonies onto nitro- cellulose filters , 1.93-1.95 Replication of bacteriophage λ , 2.5 of bacteriophage M13 , 4.4-4.6 of plasmids , 1.2-1.5 relaxed , 1.3 rolling circle , 2.4 viral replicons , 16.8-16.9 ...
... replicating bacterial colonies onto nitro- cellulose filters , 1.93-1.95 Replication of bacteriophage λ , 2.5 of bacteriophage M13 , 4.4-4.6 of plasmids , 1.2-1.5 relaxed , 1.3 rolling circle , 2.4 viral replicons , 16.8-16.9 ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector