Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-32
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 1. Digest an appropriate amount of DNA with one or more restriction enzymes . For Southern ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 1. Digest an appropriate amount of DNA with one or more restriction enzymes . For Southern ...
Page 15-8
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
... restriction sites . Enzyme ( s ) should be chosen that have potential cleavage sites distributed over the entire length of the target sequence . Often , it is necessary to use two or more restriction enzymes to obtain the desired type ...
Page 15-13
... restriction enzymes , followed by gel electrophoresis . The best approach is to identify from the restriction map of the original plasmid two restriction enzymes that each cleave the plasmid once at different locations . Ideally , these ...
... restriction enzymes , followed by gel electrophoresis . The best approach is to identify from the restriction map of the original plasmid two restriction enzymes that each cleave the plasmid once at different locations . Ideally , these ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector