Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 74
Page 9-27
... sample at 0 ° C . If all has gone well , select DNA in the size range appropriate for a particular bacteriophage A or cosmid vector by sedimentation through a 10-40 % sucrose density gradient as described below . Alternatively , if you ...
... sample at 0 ° C . If all has gone well , select DNA in the size range appropriate for a particular bacteriophage A or cosmid vector by sedimentation through a 10-40 % sucrose density gradient as described below . Alternatively , if you ...
Page 13-55
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
... samples . Excess TBE should be ejected before the next sample is drawn into the syringe and needle . • An automatic micropipettor ( 20 μl ) equipped with a standard tip . Because the tip is too large to fit between the glass plates , the ...
Page 14-23
... sample to the reservoir chamber of a Centricon 30 microconcentrator ( see Figure 14.3 ) . These concentrators are easily distinguished from other Centricon con- centrators by virtue of their clear plastic base . Do not touch the mem ...
... sample to the reservoir chamber of a Centricon 30 microconcentrator ( see Figure 14.3 ) . These concentrators are easily distinguished from other Centricon con- centrators by virtue of their clear plastic base . Do not touch the mem ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector