Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxi
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
Page 12-3
... screening of expression libraries when no nucleic acid probes are available for the gene of interest . Recently , synthetic double - stranded oligonucleotides corresponding to se- quences derived from the upstream regulatory elements of ...
... screening of expression libraries when no nucleic acid probes are available for the gene of interest . Recently , synthetic double - stranded oligonucleotides corresponding to se- quences derived from the upstream regulatory elements of ...
Page 12-16
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Immunological Screening of Expression Libraries SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE À VECTORS 1. Using a single colony of the appropriate strain of E. coli as ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Immunological Screening of Expression Libraries SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE À VECTORS 1. Using a single colony of the appropriate strain of E. coli as ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector