Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 77
Page 8-49
... sequence that have been synthesized in vitro . The sequence of these probes is deduced , using the genetic code , from short regions of the known amino acid sequence of the protein of interest . Because of the degeneracy of the genetic ...
... sequence that have been synthesized in vitro . The sequence of these probes is deduced , using the genetic code , from short regions of the known amino acid sequence of the protein of interest . Because of the degeneracy of the genetic ...
Page 13-14
... sequence required , and the facilities that are available . Only a minor proportion of projects involve the de novo accumulation of large tracts of virgin sequence . More often , sequencing is used to map and identify mutations ( e.g. ...
... sequence required , and the facilities that are available . Only a minor proportion of projects involve the de novo accumulation of large tracts of virgin sequence . More often , sequencing is used to map and identify mutations ( e.g. ...
Page 13-18
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing facilities becomes an overriding consideration when weighing the pros and cons of the ...
... sequencing project relies heavily on computer programs to sort and order the primary sequence data ( Staden 1986 ) . Access to adequate computing facilities becomes an overriding consideration when weighing the pros and cons of the ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector