Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxiii
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES 13.70 Sequencing of Plasmid DNAs Purified by ...
... Sequencing Reactions 13.61 DIDEOXY - MEDIATED SEQUENCING REACTIONS USING SEQUENASES 13.65 Preparation 13.65 Sequencing Reactions 13.67 SEQUENCING DENATURED DOUBLE - STRANDED DNA TEMPLATES 13.70 Sequencing of Plasmid DNAs Purified by ...
Page 13-42
... sequencing reactions . Before setting up sequencing reactions , it is advisable to check the amount of single - stranded template in each preparation of single - stranded bac- teriophage M13 or phagemid DNA . This can be done by ...
... sequencing reactions . Before setting up sequencing reactions , it is advisable to check the amount of single - stranded template in each preparation of single - stranded bac- teriophage M13 or phagemid DNA . This can be done by ...
Page 13-71
... sequencing reactions as described on pages 13.67-13.69 with the following modifications : • Use all of the plasmid DNA ( 2.5 μg ) in a single set of sequencing reactions . • Titrate the amount of primer . Although denatured double ...
... sequencing reactions as described on pages 13.67-13.69 with the following modifications : • Use all of the plasmid DNA ( 2.5 μg ) in a single set of sequencing reactions . • Titrate the amount of primer . Although denatured double ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector