Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-49
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
... stored at -20 ° C . The stock solution is diluted tenfold into prehybridization buffer ( usually 6 × SSC or 6 × SSPE containing 0.5 % SDS and 100 μg / ml denatured , fragmented salmon sperm DNA ) . 50 × Denhardt's reagent contains 5 g ...
Page 9-58
... stored under vacuum at room temperature until needed . REMOVING PROBES FROM NYLON MEMBRANES 1. Either • • immerse the membrane in several hundred milliliters of 1 mM Tris Cl ( pH 8.0 ) , 1 mM EDTA ( pH 8.0 ) , 0.1 × Denhardt's reagent ...
... stored under vacuum at room temperature until needed . REMOVING PROBES FROM NYLON MEMBRANES 1. Either • • immerse the membrane in several hundred milliliters of 1 mM Tris Cl ( pH 8.0 ) , 1 mM EDTA ( pH 8.0 ) , 0.1 × Denhardt's reagent ...
Page 13-48
... stored at 4 ° C for several weeks . 6 % Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a ...
... stored at 4 ° C for several weeks . 6 % Acrylamide / urea top solution 40 % acrylamide solution 5x TBE urea ( ultrapure ) 75 ml 50 ml 230 g Adjust the volume to 500 ml with deionized water , and filter the solution through a ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector