Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-15
... strand of cDNA , and almost all clones of cDNA made before 1982 were obtained using this enzymatic reaction ( see , e.g. , Efstratiadis et al ... sequences immediately adjacent to the cap Construction and Analysis of cDNA Libraries 8.15.
... strand of cDNA , and almost all clones of cDNA made before 1982 were obtained using this enzymatic reaction ( see , e.g. , Efstratiadis et al ... sequences immediately adjacent to the cap Construction and Analysis of cDNA Libraries 8.15.
Page 8-30
A Laboratory Manual Joseph Sambrook, Tom Maniatis. The synthesis of the first strand of cDNA is comparatively inefficient , since it is often difficult to incorporate sufficient dT - tailed vector in the reaction to achieve an optimal ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. The synthesis of the first strand of cDNA is comparatively inefficient , since it is often difficult to incorporate sufficient dT - tailed vector in the reaction to achieve an optimal ...
Page 8-64
... Strand of cDNA The unlabeled first - strand reaction needs no further treatment before pro- ceeding to the synthesis of the second strand of cDNA . 1. Add the following reagents directly to the first - strand reaction mixture : 10 mM ...
... Strand of cDNA The unlabeled first - strand reaction needs no further treatment before pro- ceeding to the synthesis of the second strand of cDNA . 1. Add the following reagents directly to the first - strand reaction mixture : 10 mM ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector