Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-22
... target DNA have an equal chance of being fragmented by sonication . Without ligation , monomeric linear target DNA will tend to be sheared preferentially near its center during sonication . The ligation step is less important if the DNA ...
... target DNA have an equal chance of being fragmented by sonication . Without ligation , monomeric linear target DNA will tend to be sheared preferentially near its center during sonication . The ligation step is less important if the DNA ...
Page 13-24
... Target DNA 1. Using the appropriate restriction enzymes , digest a sufficient amount of recombinant vector to yield 10–15 μg of purified target DNA . Separate the target DNA from the vector by preparative gel electrophoresis , and recover ...
... Target DNA 1. Using the appropriate restriction enzymes , digest a sufficient amount of recombinant vector to yield 10–15 μg of purified target DNA . Separate the target DNA from the vector by preparative gel electrophoresis , and recover ...
Page 13-82
... target DNA either into the Smal site lying between the two Tth1111 sites or into one of the other restriction sites that lies on either side of these sites . When the recombinant plasmid is cleaved with Tth1111 , new termini are created ...
... target DNA either into the Smal site lying between the two Tth1111 sites or into one of the other restriction sites that lies on either side of these sites . When the recombinant plasmid is cleaved with Tth1111 , new termini are created ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector