Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 77
Page 11-55
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
... target . The optimal temperature for hybridization is then determined on the basis of this value . The procedure requires a cloned target sequence that is complementary ( perfectly or imperfectly , depending on the experiment ) to the ...
Page 14-30
... target sequence from the yield of amplified product in a standard polymerase chain reaction . Recently , however , G. Gilliland , S. Perrin , and H.F. Bunn ( pers . comm . ) have devised a method to measure the overall efficiency of ...
... target sequence from the yield of amplified product in a standard polymerase chain reaction . Recently , however , G. Gilliland , S. Perrin , and H.F. Bunn ( pers . comm . ) have devised a method to measure the overall efficiency of ...
Page 14-32
... target sequence . Plot this ratio against the amount of control template added to each amplification reaction . From ... target se- quence in the reaction ) . Calculate the concentration of the target sequence in the original sample ...
... target sequence . Plot this ratio against the amount of control template added to each amplification reaction . From ... target se- quence in the reaction ) . Calculate the concentration of the target sequence in the original sample ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector