Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 10-34
... template DNA by digestion with a suitable restriction enzyme . Analyze an aliquot ( 100 ng ) of the digested DNA by agarose gel electrophoresis . If necessary , add more restriction enzyme and continue incubation until there is no trace ...
... template DNA by digestion with a suitable restriction enzyme . Analyze an aliquot ( 100 ng ) of the digested DNA by agarose gel electrophoresis . If necessary , add more restriction enzyme and continue incubation until there is no trace ...
Page 11-40
... DNA polymer- ase I to synthesize a strand of DNA complementary to the synthetic oligonu- cleotide ( Studencki and Wallace 1984 ; Ullrich et al . 1984b ) ( Figure 11.5 ) . A short primer is hybridized to an oligonucleotide template whose ...
... DNA polymer- ase I to synthesize a strand of DNA complementary to the synthetic oligonu- cleotide ( Studencki and Wallace 1984 ; Ullrich et al . 1984b ) ( Figure 11.5 ) . A short primer is hybridized to an oligonucleotide template whose ...
Page 14-14
... template DNA . This includes the mineral oil used to prevent evaporation . Then add the template DNA , close the tube , and mix the contents by gently tapping the side with a gloved finger . Centrifuge the tube briefly ( 10 seconds ) to ...
... template DNA . This includes the mineral oil used to prevent evaporation . Then add the template DNA , close the tube , and mix the contents by gently tapping the side with a gloved finger . Centrifuge the tube briefly ( 10 seconds ) to ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector