Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xix
... Termini of DNA 10.51 LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA USING THE KLENOW FRAGMENT OF E. coli DNA POLYMERASE I 10.51 LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA WITH BACTERIOPHAGE T4 DNA POLYMERASE Rapid End ...
... Termini of DNA 10.51 LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA USING THE KLENOW FRAGMENT OF E. coli DNA POLYMERASE I 10.51 LABELING THE 3 ' TERMINI OF DOUBLE - STRANDED DNA WITH BACTERIOPHAGE T4 DNA POLYMERASE Rapid End ...
Page 10-51
... Termini of DNA The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of defined size ...
... Termini of DNA The termini of DNA molecules can be radiolabeled enzymatically to yield molecules that may be used in nuclease - S1 mapping of RNA , as radiolabeled primers in primer - extension reactions , and as markers of defined size ...
Page 10-62
... TERMINI AS SUBSTRATES DNA molecules with blunt ends or recessed 5 ' termini are labeled less efficiently with bacteriophage T4 polynucleotide kinase than molecules with protruding 5 ' termini . Even a partial reaction at recessed 5 ...
... TERMINI AS SUBSTRATES DNA molecules with blunt ends or recessed 5 ' termini are labeled less efficiently with bacteriophage T4 polynucleotide kinase than molecules with protruding 5 ' termini . Even a partial reaction at recessed 5 ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector