Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xxvi
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
... Transfection of Cells Growing in Suspension 16.38 Modified Calcium Phosphate - mediated Transfection Procedure 16.39 TRANSFECTION MEDIATED BY DEAE - DEXTRAN 16.41 Transfection Using DEAE - Dextran : Protocol I 16.42 Transfection Using ...
Page 15-59
... transfection . The aim of this step was to reduce the background of plaques containing wild - type target DNA . However , this time - consuming enrichment procedure is no longer necessary because of the improvement in efficiency of ...
... transfection . The aim of this step was to reduce the background of plaques containing wild - type target DNA . However , this time - consuming enrichment procedure is no longer necessary because of the improvement in efficiency of ...
Page 1-26
... transfection . See Transfection , of mammalian cells transient expression in COS cells , 16.17 , 16.19-16.22 viral replicons , 16.8-16.9 genomic DNA agarose gel electrophoresis , 9.32-9.33 . See also Pulsed - field gel electro- phoresis ...
... transfection . See Transfection , of mammalian cells transient expression in COS cells , 16.17 , 16.19-16.22 viral replicons , 16.8-16.9 genomic DNA agarose gel electrophoresis , 9.32-9.33 . See also Pulsed - field gel electro- phoresis ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector