Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 81
Page 12-38
... ml . 2. Grow a 2 - ml culture of E. coli strain Y1089 to saturation in LB medium containing 0.2 % maltose , 50 μg / ml ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 ...
... ml . 2. Grow a 2 - ml culture of E. coli strain Y1089 to saturation in LB medium containing 0.2 % maltose , 50 μg / ml ampicillin , and 10 mM MgCl2 . 3. Dilute 50 μl of the saturated culture with 2 ml of LB medium containing 10 mM MgCl2 ...
Page 13-44
... μg / ml 284 μg / ml 0.5 mм dGTP 0.5 mм dTTP 304 μg / ml ( or 0.5 mм dITP 288 μg / ml ) 291 μg / ml Stock solutions of ddNTPs contain a single ddNTP at a concentration of 10 mм in TE ( pH 8.0 ) : 10 mм ddATP 10 mм ddCTP 10 mм ddGTP 10 mм ...
... μg / ml 284 μg / ml 0.5 mм dGTP 0.5 mм dTTP 304 μg / ml ( or 0.5 mм dITP 288 μg / ml ) 291 μg / ml Stock solutions of ddNTPs contain a single ddNTP at a concentration of 10 mм in TE ( pH 8.0 ) : 10 mм ddATP 10 mм ddCTP 10 mм ddGTP 10 mм ...
Page 15-46
... μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C . 11. From the numbers of colonies obtained ... μg / ml . Incubate the plates for 18-24 hours at 37 ° C . 13. At the end of the incubation , add to each plate ...
... μg / ml ) . Store the remainders of the ligation mixtures at -20 ° C . 11. From the numbers of colonies obtained ... μg / ml . Incubate the plates for 18-24 hours at 37 ° C . 13. At the end of the incubation , add to each plate ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector