Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 83
Page 8-47
... Usually , a fragment derived from one end or the other of the existing clone is isolated , radiolabeled in vitro , and used to probe a library . Hybridization with homologous probes is always carried out under stringent conditions ...
... Usually , a fragment derived from one end or the other of the existing clone is isolated , radiolabeled in vitro , and used to probe a library . Hybridization with homologous probes is always carried out under stringent conditions ...
Page 11-48
... Usually , conditions are chosen to be 2 ° C below the calculated T. of the most A / T - rich member of the pool ( Suggs et al . 1981b ) . However , the use of such " lowest common denominator " conditions can lead to a number of false ...
... Usually , conditions are chosen to be 2 ° C below the calculated T. of the most A / T - rich member of the pool ( Suggs et al . 1981b ) . However , the use of such " lowest common denominator " conditions can lead to a number of false ...
Page 14-15
... ( usually 72 ° C ) . It is assumed that the Taq DNA polymer- ase begins to work , albeit sluggishly , as soon as the ... Usually , oligonucleotides are used at a concentration of 1 μм in polymerase chain reactions . This is usually ...
... ( usually 72 ° C ) . It is assumed that the Taq DNA polymer- ase begins to work , albeit sluggishly , as soon as the ... Usually , oligonucleotides are used at a concentration of 1 μм in polymerase chain reactions . This is usually ...
Other editions - View all
Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector