Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-38
... volume of 100 μl or less ) on the column . Immediately after the sample has entered the column , add 100 μl of ... volume of phenol : chloroform . Reextract the organic phase with 50 μl of 10 mM Tris · Cl ( pH 8.0 ) , and combine the two ...
... volume of 100 μl or less ) on the column . Immediately after the sample has entered the column , add 100 μl of ... volume of phenol : chloroform . Reextract the organic phase with 50 μl of 10 mM Tris · Cl ( pH 8.0 ) , and combine the two ...
Page 11-42
... volume as possible . It is therefore better to use as substrates radiolabeled dNTPs supplied in ethanol / water rather than those supplied in buffered aqueous solvents . Appropriate volumes of the ethanolic [ a - 32P ] dNTPs can be ...
... volume as possible . It is therefore better to use as substrates radiolabeled dNTPs supplied in ethanol / water rather than those supplied in buffered aqueous solvents . Appropriate volumes of the ethanolic [ a - 32P ] dNTPs can be ...
Page 13-84
... volume of dimethyl sulfate with 9 volumes of ethanol . 50 mM sodium cacodylate ( pH 7.0 ) 1 mM EDTA ( pH 8.0 ) 1.5 M sodium acetate ( pH 7.0 ) 1 M ẞ - mercaptoethanol 250 μg / ml yeast tRNA Chill to -20 ° C . 95 % ( Eastman Kodak ...
... volume of dimethyl sulfate with 9 volumes of ethanol . 50 mM sodium cacodylate ( pH 7.0 ) 1 mM EDTA ( pH 8.0 ) 1.5 M sodium acetate ( pH 7.0 ) 1 M ẞ - mercaptoethanol 250 μg / ml yeast tRNA Chill to -20 ° C . 95 % ( Eastman Kodak ...
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Common terms and phrases
agarose gel aliquots amplification annealing antibody bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter cleavage cleaved cleotide codon coli DNA polymerase concentration containing deletions denatured digestion dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Incubate inserted Klenow fragment ligation linear linkers method microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filters Nucleic Acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probes purified radioactivity radiolabeled reaction mixture recombinant remove restriction enzyme reverse transcriptase room temperature samples screening sequencing reactions single-stranded DNA solution step Store strand of cDNA synthesis target DNA target sequence template DNA termini transfer Tris Cl pH vector