Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xii
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
... DNA Mapping 5.26 DIGESTING DNA WITH RESTRICTION ENZYMES 5.28 Setting Up Digestions with Restriction Enzymes 5.31 Other Enzymes Used in Molecular Cloning 5.33 DNA POLYMERASES 5.35 DNA Polymerase I ( Holoenzyme ) 5.36 Large Fragment of DNA ...
Page F-2
... polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends ... DNA polymerase F.2 Subcloning FILLING RECESSED 3' TERMINI F 2 I.
... polymerase activity of the Klenow fragment of E. coli DNA polymerase I in the presence of the appropriate dNTPs . Which of the four dNTPs are added to the reaction depends ... DNA polymerase F.2 Subcloning FILLING RECESSED 3' TERMINI F 2 I.
Page F-4
... DNA polymerase I and bacteriophage T4 DNA polymerase carry a 3'5 ' exonuclease activity that can be used to remove protruding nucleotides from the 3 ' termini of DNA . Both enzymes lack a 5 ' → 3 ' exonuclease activity and carry a 5 ...
... DNA polymerase I and bacteriophage T4 DNA polymerase carry a 3'5 ' exonuclease activity that can be used to remove protruding nucleotides from the 3 ' termini of DNA . Both enzymes lack a 5 ' → 3 ' exonuclease activity and carry a 5 ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml