Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 96
Page 17-43
... coli . Gene 42 : 175 . Marston , F.A.O. 1987. The purification of eukaryotic polypeptides expressed in Escherichia coli . In DNA cloning : A practical approach ( ed . D.M. Glover ) , vol . 3 , p . 59. IRL Press , Oxford . Marston ...
... coli . Gene 42 : 175 . Marston , F.A.O. 1987. The purification of eukaryotic polypeptides expressed in Escherichia coli . In DNA cloning : A practical approach ( ed . D.M. Glover ) , vol . 3 , p . 59. IRL Press , Oxford . Marston ...
Page 1-18
... coli strain , 4.15 , A.13 JM107 , E. coli strain . genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , 4.15 , A.13 JM108 , E. coli strain , 4.15 , A.13 JM109 , E. coli strain genotype and uses , A.10 use in ...
... coli strain , 4.15 , A.13 JM107 , E. coli strain . genotype and uses , A.10 use in propagation of bacteriophage M13 vectors , 4.14 , 4.15 , A.13 JM108 , E. coli strain , 4.15 , A.13 JM109 , E. coli strain genotype and uses , A.10 use in ...
Page 1-37
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 recA ̄ strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
... coli strain , genotype and uses , 17.13 , A.11 recA , genetic marker in E. coli host strain , 2.57 , 3.6 , 4.13 RecA protein , characteristics and use , 5.88 recA ̄ strains of E. coli , 3.6 , 3.34 . See also E. coli , strain list recB ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml