Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-46
... efficiency . In these cases , it is best to transfect large monolayers of cells ( 90 - mm dishes ) and then to ... efficiency of stable transformation , which can vary over several orders of magnitude ( see , e.g. , Spandidos and Wilkie ...
... efficiency . In these cases , it is best to transfect large monolayers of cells ( 90 - mm dishes ) and then to ... efficiency of stable transformation , which can vary over several orders of magnitude ( see , e.g. , Spandidos and Wilkie ...
Page 16-54
... efficiency of transfection has varied widely in reports from different laboratories . Recently , two groups have examined the parame- ters that affect transfection efficiencies and have shown that different condi- tions must be used for ...
... efficiency of transfection has varied widely in reports from different laboratories . Recently , two groups have examined the parame- ters that affect transfection efficiencies and have shown that different condi- tions must be used for ...
Page 16-73
... efficiency COS cell expression system . Proc . Natl . Acad . Sci . 84 : 8573 . Bacchetti , S. and F.L. Graham . 1977 . Transfer of the gene for thymidine ki- nase to thymidine kinase - deficient human cells by purified herpes simplex ...
... efficiency COS cell expression system . Proc . Natl . Acad . Sci . 84 : 8573 . Bacchetti , S. and F.L. Graham . 1977 . Transfer of the gene for thymidine ki- nase to thymidine kinase - deficient human cells by purified herpes simplex ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids ammonium persulfate amount antibody antigen aqueous assay autoclaving bacterial BamHI bromophenol blue cDNA centrifugation coli column containing culture deionized H₂O diluted Dissolve dithiothreitol DNA polymerase dNTPs EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide eukaryotic expression extract film fragment hybridization Incubate lb/sq ligation linkers liquid cycle lysate mammalian cells medium method mg/ml MgCl2 microfuge tube minutes at 15 minutes at 4°C minutes at room mixture mRNAs NaCl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction reagent remove restriction enzyme room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequences sodium Sterilize by autoclaving stock solution stored strain supernatant target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml